increases the chance of a healthy birth per embryo transfer.

Best-in-class
accuracy
A high number of euploid embryos reported
Improved clinical outcomes
Fewer embryos classified mosaic

The best-in-class accuracy of PGT[A]Seq means an increased number of euploid embryos are correctly reported, leading to more viable embryos being transferred with higher pregnancy rates than is achieved using less accurate PGT-A methods.

More embryos are reported ‘euploid’ using PGT[A]Seq compared to some other PGT-A methods

  • More cycles have an embryo transfer
  • More cycles achieve a pregnancy
Advanced
next generation sequencing method

Unlike most other methods, PGT[A]Seq combines two distinct pieces of information to provide unparalleled accuracy. The test simultaneously assesses the quantity of DNA at thousands of sites on each chromosome using an advanced next generation sequencing method. The test also evaluates numerous variations in the DNA sequence, known as single nucleotide polymorphisms (SNPs). Together, these two pieces of information provide major advantages over other PGT-A methods, including the detection of triploid embryos. The PGT[A]Seq technology is the most accurate test of its kind, with proven clinical validity.

- The most powerful embryo selection tool

- The most accurate and highly validated test of its kind

PGT[A]Seq method provides two pieces of information:

NGS

Quantity of DNA measured at thousands of individual sites

SNPs

Genotypes determined for thousands of DNA polymorphisms

Clinical impact

- Avoid unsuccessful transfer of non-viable aneuploid embryos

- Reduced miscarriage rate

- Reduced risk of aneuploid syndromes

- Faster time to pregnancy

- Permits high efficiency single embryo transfer (SET)

- Accurate detection of triploidy

- Avoids incorrect 'mosaic' designation

- Higher proportion of embryos classified 'euploid'

PGT[A]Seq

by Juno

The most powerful and accurate embryo analysis on the market

Juno uses a unique PGT[A]Seq methodology, which has successfully demonstrated clinical validity in a non-selectin study

Our PGT[A]Seq technique employs the latest next-generation sequencing methods, increasing the quality of the data obtained and yielding results of unparalleled accuracy

How does it work?

01
DNA amplified from all 24 chromosomes
02
Matching fragments of DNA with the chromosomes they came from
03
Measurement of the quantity of DNA for each chromosome

1 NGS

Juno uses next-generation sequencing to measure the amount of DNA at thousands of sites on each chromosome. This allows the number of copies of the chromosome to be calculated with high accuracy.

2 SNPs

Juno looks at thousands of places where the DNA sequence can differ between individual chromosomes. Each of these sites of variation can be type ’A’ or type ‘B’. Normal, Trisomy and monosomy each have characteristic patterns of As and Bs.
Together, the measurement of the amount of DNA and the analysis of the DNA sequence greatly increases the accuracy of PGT[A]Seq
PGTA_logo
PROCESS

Biopsy and embryo vitrification

PGT[A]Seq

RESULTS

5-10 days

Transfer of normal frozen embryos

Biopsy and embryo vitrification

PGT-[A]Seq

RESULTS

5-10 days

Transfer of normal frozen embryos

Juno vs other labs

other labs
JUNO
25,007 embryos analyzed.
%transferable per informative embryo
(mosaic are included in the Juno column)

REDUCTION IN

MOSAIC

EMBRYOS

The enhanced accuracy of PGT[A]Seq prevents viable embryos from being incorrectly classified mosaic.
0 %
MORE EUPLOID EMBRYOS
+ 0
EXTRA EMBRYOS reported for transfer from a dataset of 25,007 embryos derived from 15 different IVF clinics
+ 0
EMBRYOS AVAILABLE to Transfer

Know your
Clinical Rates

1.5%

NON
INFORMATIVE

EMBRYOS

Juno PGT[A]Seq

Fewer mosaic embryos reported

A high-profile study involving the most widely used PGT[A]Seq method, performed at nine genetic laboratories in four different countries, reported a high incidence of mosaicism (Munne et al., 2019). Juno’s PGT[A]Seq method is highly validated for the detection of aneuploidy and mosaicism in embryo biopsy specimens. PGT[A]Seq avoids the incorrect classification of potentially viable embryos as “Mosaic” or “abnormal”.

Advantages of using the Juno PGT[A]Seq

01
The most powerful embryo selection tool currently available
06
Provides information on likely potential of stored material and avoids storage of non-viable embryos
02
High accuracy, including detection of triploid embryos and detection of DNA contamination
07
Reduced miscarriage rate
03
Avoid unsuccessful transfer of non-viable aneuploid embryos
08
Reduced risk of aneuploid syndromes
04
Permits high efficiency single embryo transfer (SET)
09
Avoids incorrect classification of euploid embryos as abnormal or mosaic
05
Faster time to pregnancy
10
Compared to other methods, Juno PGT[A]Seq is associated with a higher proportion of embryos categorised as euploid
01
The most powerful embryo selection tool currently available
02
High accuracy, including detection of triploid embryos
03
Avoid unsuccessful transfer of non-viable aneuploid embryos
04
Permits high efficiency single embryo transfer (SET)
05
Faster time to pregnancy
06
Provides information on likely potential of stored material and avoids storage of non-viable embryos
07
Reduced miscarriage rate
08
Reduced risk of aneuploid syndromes
09
Avoids incorrect classification of euploid embryos as abnormal or mosaic
10
Compared to other methods, Juno PGT-[A]Seq is associated with a higher proportion of embryos categorised as euploid

Who should use PGT[A]Seq?

All couples undergoing IVF have a high likelihood that some of their embryos will be aneuploid. Such embryos typically fail to implant, miscarry, or more rarely produce abnormal pregnancies affected by problems such as Down syndrome. Consequently, PGT[A]Seq is likely to have value for all couples. However, testing chromosomes in embryos becomes progressively more important as women age. By the age of 35 it is typical for more than one-third of embryos to be aneuploid, and by the time a woman is over 40, more than three-quarters of embryos are chromosomally abnormal.

Chromosome abnormalities are invisible without using specially designed tests. PGT-A[seq] is the most accurate method for distinguishing embryos that have the potential to form a healthy pregnancy from embryos that are not viable due to aneuploidy. Identifying the embryos that are most likely to produce a pregnancy, and ensuring they are prioritized for transfer to the uterus, helps maintain the highest possible pregnancy rates when using single embryo transfer (SET) strategies to reduce the incidence of multiple gestations.

PGT[A]Seq results are typically available 6 days after Juno receives the embryo biopsy samples (although results can take up to 10 days).

We aim to make working with Juno Genetics as easy as possible. Juno is committed to supporting the clinics it works with, providing guidance at all stages of the PGT process. We are available seven days a week to assist with queries or to provide advice. At Juno we appreciate that different clinics may have unique needs and our policy is one of flexibility, where we adapt to the specific requirements of each clinic. If your clinic already offers PGT in collaboration with another genetics laboratory, then it is likely that very few significant changes, if any, will be necessary. Please contact Juno Genetics and we will be happy to walk you through the enrolment procedure, provide all necessary documentation and answer any questions you might have. If your clinic is new to PGT, Juno can help by providing valuable information and documentation to get you started. Juno can also assist with training and evaluation of procedures, making sure that all elements of the PGT procedure are optimised and working well, prior to the launch of a clinical service. Please contact supportuk@junogenetics.com to begin working with Juno.

If the proportion of samples that fail to give a PGT result is consistently higher than expected, this is almost always due to a problem with technique used for the cell washing and loading or, less commonly, with the embryo biopsy method. Scientists at Juno Genetics have decades of experience working with clinics in order to achieve optimal PGT results and can assist with troubleshooting. Problems are almost always solved after simple and easy to implement changes to procedure.

900 828 420